| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection
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| Autores: |
Le Bris, Cedric
Richard, Gaelle
Paillard, Christine
Lambert, Christophe
Seguineau, Catherine
Gauthier, Olivier
Pernet, Fabrice
Guerard, Fabienne
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| Data: |
2015-01
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| Ano: |
2015
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| Palavras-chave: |
Venerupis philippinarum
Vibrio tapetis
Immunity
Phenoloxidase
Superoxide dismutase
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| Resumo: |
Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection. This first response in the mantle could be explained by the proximity to the injection region (extrapallial cavity). In the hemolymph the response occurred at later times and was associated with an increase in PO activity and a decrease in SOD activity. As hemolymph is a circulating fluid, this response delay could be due to an “integration time” needed by the organism to counteract the infection. Injections also impacted PO and SOD activities in both tissues and confirmed a difference in pathogenicity between the two V. tapetis strains.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00221/33257/31733.pdf
DOI:10.1016/j.fsi.2014.10.021
https://archimer.ifremer.fr/doc/00221/33257/
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| Editor: |
Academic Press Ltd- Elsevier Science Ltd
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| Formato: |
application/pdf
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| Fonte: |
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2015-01 , Vol. 42 , N. 1 , P. 16-24
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| Direitos: |
2014 Elsevier Ltd. All rights reserved.
info:eu-repo/semantics/openAccess
restricted use
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